Webb1 mars 2014 · The 1.3-kb SK gene was PCR amplified using gene-specific primers SK F1 and SK R1 (Table 1), with an annealing temperature of 53 °C.The forward primer contained an NdeI site (in italics), and the reverse primer contained an EcoRI site (in italics).The PCR amplicons were digested with the restriction enzymes NdeI and EcoRI and then ligated … Webb1 mars 2014 · SK is a single-chain polypeptide containing 414 amino acids with a molecular mass of 47,000. It consists of 3 domains: α, β and γ (Malke and Ferretti, 1984). SK by itself is not a plasminogen activator; it forms a 1:1 stoichiometric complex with plasminogen known as the streptokinase–plasminogen activator complex.
pRSET A, B, & C Bacterial Expression Vectors - Thermo …
http://www.biofeng.com/zaiti/dachang/pRSETB.html Webb5 juli 2016 · In short, they were cloned into the pRSETB vector (Invitrogen, Carlsbad, CA, USA), containing an N-terminal HIS-tag with an additional X-press epitope, and generated in E. coli Rosetta Gami (DE3) plysS. Expression was induced with 1 mM Isopropyl β-d-1-iogalactopyranoside (IPTG). fh39 hose
Addgene: pRSetB-KanR-LiLac
Webberated NcoI and EcoRI sites that allowed subcloning into pRSETB vector (Invitrogen) to generate pRSETB-MHRI for prokaryotic expres-sion of recombinant mHRI polyhistidine-tagged under the control of the T7 promoter. For the expression of mHRI in mammalian cells under the control of a cytomegalovirus promoter, the PCR oligonucleotides gener- WebbInitially, skc gene was cloned in the pRSETB vector and it is designated as pRSETB-SK. To understand the effect of rare codon AGG, it was inserted at +2, +3 and +5 positions of. Webbvector (Invitrogen). The GGBP–cpYFP insertion variants were constructed by overlap PCR19 using wild-type GGBP sequence and cpYFP from Frex.14 First, the coding … denver sushi house