WebNorthern blots showed a major effect of enolase and a minor effect of SR7P on the half-life of rpsO mRNA indicating a fine-tuning role of SR7P in RNA degradation. Keywords: bacillus subtilis; Dual-function antisense RNA; RNA degradation; degradosome; enolase; regulatory peptide; rnase Y; sr7; sr7p. Publication types Research Support, Non-U.S. Gov't WebFeb 25, 2024 · For rough evaluation of 4b-Mango binding constants, 10 nM solution of 4b in the working buffer supplemented with 0.05% Tween-20 was titrated with increasing concentrations of Mango RNA, and integral fluorescence was registered in microcapillaries using Monolith NT.115 device (NanoTemper, Germany) equipped with a RED/GREEN …
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