WebApr 4, 2024 · Inject i.v. 400 ng of PTx in 200 μL of saline into the tail vein of mice using an insulin syringe on days 0, 2, and 7. Assess clinical scores of EAE daily with a 0–5 scoring system (0, no signs; 1, flaccid tail; 2, impaired righting reflex and/or gait; 2.5, paralysis of one hind limb; 3, paralysis of both hind limbs; 4, hind limb paralysis with partial fore limb … WebSIGMA-ALDRICH sigma-aldrich.com SAFETY DATA SHEET Version 5.0 Revision Date 07/04/2015 Print Date 11/20/2024 1. PRODUCT AND COMPANY IDENTIFICATION 1.1 …
Phosphate-buffered saline (PBS, 1X), sterile-filtered
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SIGMA Phosphate buffered saline, tablet - Thomas Sci
WebSep 17, 2024 · After dispensing 15,000 HS746T and 15,000 NCIN87 per well in a 12-well plate, the next day, Chloroquine , Sigma), hydroxychloroquine sulfate (Sigma), and mefloquine hydrochloride (R&D System) were treated at concentrations of 1, 2.5, 5, 10, 25, 50 or 100 ug/ml, respectively. 48 hours after the treatment, the cell culture medium was … WebAn aliquot of 190 µL of the cell suspension was mixed with 10 µL of propidium iodide solution (Abcam, Cambridge, UK), and the resulting mixture was analyzed by FC. hAMSCs were preliminarily harvested with 0.05% trypsin (PanEco, Moscow, Russia) in 0.53 mM EDTA (Sigma-Aldrich, St. Louis, MO, USA) and washed twice with phosphate-buffered saline. WebThe drug release profile showed minimal imatinib leakage (<5%) in phosphate-buffered saline (PBS) at pH =7.4 within 72 hours of ... Roswell Park Memorial Institute medium … small fry animal crossing